Human D2-Hydroxyglutarate Dehydrogenase (D2HGDH) ELISA Kit from MyBioSource.com

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Human D2-Hydroxyglutarate Dehydrogenase (D2HGDH) ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of human D2-Hydroxyglutarate (also known as D2HGDH) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human D2HGDH antibody. D2HGDH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human D2HGDH Antibody is added and binds to D2HGDH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated D2HGDH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human D2HGDH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm